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Which test tubes were used to determine the optimal ph for lipase activity?

Which test tubes were used to determine the optimal ph for lipase activity?
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Which test tubes were used to determine the optimal ph for lipase activity?

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User SteelBytes
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The tubes 1, 5 and 6 is the answer to the question which test tubes were used to determine the optimal ph lipase activity. Lipase is an enzyme and optimum ph the maximum possible point on which enzyme become active.
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User Dheeraj D
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