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What do we now have to do to successfully use a cloned gene to transform mice?

2 Answers

4 votes

Final answer:

To successfully use a cloned gene to transform mice, the gene must be inserted into a vector, which is then inserted into bacteria for transformation. The transformed bacteria are grown to produce the desired protein.

Step-by-step explanation:

To successfully use a cloned gene to transform mice, several steps must be followed. First, the gene of interest is inserted into a vector, such as a plasmid. Then, the recombinant vector is inserted into bacteria, such as E. coli, through a process called transformation. The bacteria are then selected and grown to produce the protein of interest. Finally, the protein can be used for various purposes, such as medicine or research.

For example, scientists have used mice extensively for expressing and studying recombinant genes and mutations. Mice can be genetically modified to express transgenic DNA and produce desired proteins.

In summary, to successfully use a cloned gene to transform mice, the gene must be inserted into a vector, which is then inserted into bacteria for transformation. The transformed bacteria are grown to produce the desired protein.

answered
User KennyB
by
8.2k points
5 votes

To transform the mice, it can be done by genetic alteration and engineering which is the use of DNA insertion process either with the use of the current CRISPR technology or needle insertion. The use of electroporation. And lastly, inject a strong virus to the mice.

answered
User Ganye
by
8.0k points
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