The correct answer to this open question is the following.
What caused trouble between Virginia’s farmers and Native Americans was that Virginia colonists that were farmers took over more land to grow crops, mainly tobacco, and other colonists wanted to push their settlements forward in territories that Native American Indians considered of their property.
When Virginia Colonists Jhon Rolfe had success planting tobacco seeds from the Caribbean island of Trinidad, tobacco became the king of crops in the colony of Virginia and farmers needed more land to grow more tobacco.