Question

PLEASE HELP ASAP!!!!

How can the length of a DNA fragment the estimated in gel electrophoresis?
A. Larger fragments travel faster and father than smaller fragments.
B. Smaller fragments travel faster and farther than larger fragments.
C. Larger fragments are more negatively charged so they travel farther.
D. Smaller fragments or more negatively charged so they travel farther.

Answer 1

B. Smaller fragments travel faster and farther than larger fragments.

Explanation: Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. [2] Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins.