Question

Where would a probe with the sequence aatcg bind to a target dna with the sequence ttttagccatttacgattaatcg (recall that dna sequences are always written 5' to 3')? view available hint(s) where would a probe with the sequence aatcg bind to a target dna with the sequence ttttagccatttacgattaatcg (recall that dna sequences are always written 5' to 3')? it would not bind the target dna. ttttagccatttacgattaatcg ttttagccatttacgattaatcg ttttagccatttacgattaatcg?

Answer 1

If the DNA sequence is: 5’ TTTTAGCCATTTACGATTAATCG 3’
The probe 5’ AATCG 3’ will bind for the CGATT part of DNA.
The two complementary strands of DNA are usually differentiated as the "sense" strand (5’-3’) and the "antisense" strand (3’-5’), meaning that they have different directions. According to this, the probe in antisense would be 3’ GCTAA 5’.