Answer: Denaturation
Step-by-step explanation:
Denaturation can be defined as the process which is separation of the double strand of DNA into two parts which occurs when the hydrogen bonds between the two strands are broken.
This occurs at high temperature. In case of denaturation of nucleic acids the temperature is high. The temperature is 95 degree Celsius for 30 seconds.
This is an important step of whole PCR which consists of 3 steps. Denaturation, Annealing and Extension.