Question

Which procedure would help ensure that a foreign DNA fragment could easily incorporate into a bacterial plasmid when the two are mixed together?

a)The fragment and plasmid are both cut with the same restriction enzyme.
b)The plasmid is modified to contain GFP.
c)The plasmid is pretreated with DNA ligase.
d)The DNA fragment is pretreated with DNA ligase.

Answer 1

The correct answer is a !!

Both should be cut with same restriction enzyme to have sticky ends so that they easily combine with each other !