Answer;
- A batch culture
A closed system, used for bacterial growth, where nothing is added and nothing is removed is called a batch culture.
Explanation;
-Batch culture is a technique used to grow microorganisms or cells. A limited supply of nutrients for growth is provided; when these are used up, or some other factor becomes limiting, the culture declines. Cells, or products that the organisms have made, can then be harvested from the culture.
-In this culture, certain volume of medium is inoculated with cells. The cells grow in the medium and are exposed to changing conditions, as nutrients are depleted and waste products accumulate.