Question

Which colonies (blue, white, or both) would you want to pick for further analysis to check for the successful cloning of the antithrombin gene? briefly explain your answer?

Answer 1

You should pick both colonies for the further analysis. Colonies are white or blue depending on their possibility to metabolize X-gal product (the blue colonies can metabolize X-gal product, and they encode for lacZgene). Whether it encodes or lacks the lacZ gene, it can still uptake the antithrombin gene and grow on the medium through electroporation.