Question

When performing a transformation reaction using pBlu, you decide to also add a negative control in which no vector was added to your competent cells before heat shock. When plating onto LA Amp, you realize that the negative control plate has 230 colonies. What is a possible reason for this unexpected growth?

A.You forgot to add antibiotics to the plate.
B.You accidentally added vector to the cells.
C.You contaminated you competent cells with.
D.You added too much antibiotic to the plate.

Answer 1

A.You forgot to add antibiotics to the plate. ✔

B.You accidentally added vector to the cells.✔

C.You contaminated you competent cells with.✔

D.You added too much antibiotic to the plate.❌❌❌