Question

Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (GFP) and the insertion of the plasmid into a bacterial cell by placing the steps in order

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-plate the bacterial cells, and screen for positive transformants

Answer 1

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid

-transform bacteria with the recombinant plasmid using electroporation

-plate the bacterial cells, and screen for positive transformants