Question

In the heterozygous BRCA2 mutation of the simulation, which of the following statements is TRUE regarding the Sanger sequencing method detection of the mutation? Both the mutation and the wild-type base would be marked with the same ddNTP at that position. The dye-labeled nucleotides ONLY attach to mutated DNA, so the mutation would be easily identified. You cannot tell where the mutation is by Sanger sequencing if the individual is heterozygous for the mutation. Another technique must be used. The mutation is not labeled with a ddNTP and is therefore different from the wild-type sequence. In the sequencing reaction, the fragment identifying the mutation and the fragment identifying the wild-type base at that position would be the same length.

Answer 1

It requires a primer for the polymerase; the primer is radiolabelled so the sequences generated can be visualized.

Step-by-step explanation: