Question

Identify the mRNA sequence and orientation

if the nontemplate strand has the sequence
5' ATGCCAGTCATC 3,

Answer 1

The non- template strand will have the same sequence as the mRNA except, Thymine, T, will be replaced by Uracil, U, in the mRNA. The orientation will also be the same.

Hence, the mRNA sequence will be

5' AUGCCAGUCAUC 3'

This is because both the non- template strand and the mRNA are complementary to the template strand. Hence, they will have the same orientation as well as sequence except for thymine and uracil.