Question

Which of the following statements about PCR is/are FALSE? There may be more than one correct answer.

A) PCR reactions contain DNA template, primers, deoxyribonucleotide triphosphates, and heat- resistant DNA polymerase
B) Each PCR cycle includes three steps: denaturation, annealing, and extension
C) After each PCR cycle, the amount of DNA increases in a linear manner
D) One limitation of PCR is that it requires prior knowledge of the sequence to be amplified
E) PCR is a very sensitive technique and can amplify target sequences present in extremely low copy number
F) The single-stranded DNA primers must anneal to opposite strands of template DNA at distant sites, with their 5′ ends directed toward each other

Answer 1

Answer: The answer is C

Step-by-step explanation:

After each PCR cycle, the amount of DNA does not increase in a linear manner