Question

You are performing an in vitro test in which you are testing the adsorption of three proteins (X, Y, and Z) to a material. After you have exposed the proteins to the material surface, how do you determine which proteins adsorbed?

Answer 1

Proteins have different molecular weight, shape, size and isolelectric point. All these characteristics can be used for the separation and identification of the proteins.

The proteins X,Y and Z can be analyzed by using the HPLC ( high performance liquid chromatography) or western blot assay. The concentration of the proteins in the solution is compared with the unused control. The missing content from the solution will be present on material. The material surface can further be analyzed by the fluorescent assay or colometric assay. The proteins absorb different lights at different wavelength.