Question

The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution?Select one:

Answer 1

The concentration of dye is 2.29 mg/mL.

Step-by-step explanation:

According to Beer's law the absorbance (A) of a solution is proportional to its concentration (c). The best fit curve line to an A vs. c plot has a slope of 0.1815 mL/mg and intercept of 0.0477. The resulting equation is:

A = 0.0477 + 0.1815 mL/mg . c

If a solution has an absorbance of 0.464, the concentration of dye will be:

0.464 = 0.0477 + 0.1815 mL/mg . c

c = 2.29 mg/mL