Answer:
The main difference is the presence of a nucleus.
Step-by-step explanation:
One of the biggest differences is the presence of a nucleus with an envelope. This is very important because in eukaryotes it is present, but in prokaryotes, the genetic material is in the cytosol, although it's not exactly dispersed, it's slightly grouped. Also, the DNA in prokaryotes is organized in circular chromosomes while in eukaryotes it's organized in linear chromosomes arranged in an X shape. There are many more differences, but these two are the more important ones.