Limitations of dichotomous keys:
1. It is only useful when the organism that is being identified was or is a part of organisms that are part of the key. An organisms is usually misidentified if it does not belong to the key. 
2. It’s a complex mechanism of organism identification and may leads to chain of worng interpretations. 
3. The characteristics which are devised to identify an organism might not be a distinct feature of the species already exiting in the dichotomous key.
4. Dependency on physical characteristics, does not take into account genetic characteristics.