Question

Calculate the volume of compound X needed to ma ke a 15 ug/ml treatment solution in 0.1 L of 1x PBS using a stock solution that contains 10 ml of compound X at a concentration of 250 mg/ml. 60 ul 6 ml

Answer 1

6 μL

Step-by-step explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2, where C is the concentration, V the volume, 1 is the initial solution and 2 the final solution.

The final concentration must be 15 μg/mL with a volume of 0.1L or 100 mL. The concentration of the stock solution is 250 mg/mL. 1 mg has 1000 μg, so the concentration is 250000 μg/mL. Then:

250000xV1 = 15x100

V1 = 15/2500

V1 = 0.006 mL = 6 μL