Question

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the
buffers. Why?

Answer 1

To reduce thiol-groups of the protein

Step-by-step explanation:

Dithiothreitol of b-mercaptoethanol act as reducing agents. Including them in a buffer would prevent oxidation of thiol-groups in amino acids of the protein and preventing its denaturation.