Question

Suppose that a thin-layer chromatography plate the solvent front migrates a distance of 14 cm, and two steroid spots move distances of (a) 6 cm and (b) 12 cm. Calculate the Rf values for each steroid.

Answer 1

A)
`RF = 0.428`

B)
`RF = 0.857`

Step-by-step explanation:

RF value in thin-layer chromatography plate is defined as the ratio of distance moved by the steroid spot (A) to the distance migrated by solvent front(B)

Hence, mathematically

`RF = (A)/(B)` ------Equation (A)

a) Here
`A = 6` cm and
`B = 14`cm

Substituting the given values in above equation, we get -

`= (6)/(14) \\= 0.428\\`

b) Here
`A = 12` cm and
`B = 14`cm

Substituting the given values in above equation, we get -

`= (12)/(14) \\= 0.857\\`