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4 votes
How does crystal violet enhance the visualization of bacterial structure(s)?

asked
User Offbyone
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1 Answer

2 votes

The crystal violet molecules are trapped in the peptidoglycan layer of bacteria. For Gram-positive with a large peptidoglycan layer, the crystal violet molecules don't get washed off easily by the ethyl alcohol decolorizer (as it does in gram-negative bacteria) after staining. This makes it is possible to view the structure of the gram-positive bacteria after gram staining.

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User Airith
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