Question

Arrange the following steps from a typical genetic modification procedure into their correct sequence.

a. Select the gene of interest + insert into plasmid
b. Protein product harvested from bacteria
c. Isolate bacterial plasmid
d. Bacteria replicate
e. Cloned gene harvested from bacteria
f. Plasmid taken up by bacterium

Answer 1

c. Isolate bacterial plasmid

a. Select the gene of interest + insert into plasmid

f. Plasmid taken up by bacterium

d. Bacteria replicate

e. Cloned gene harvested from bacteria

b. Protein product harvested from bacteria

Step-by-step explanation:

Genetic modification refers to the biological procedure involving the manipulation of an organism's genome in order to achieve a desirable result. This process is carried out by inserting or deleting a desirable gene (encodes the desirable trait) from the organism.

Bacteria DNA called PLASMID is commonly used for genetic engineering/modification procedure. The plasmid is used to convey/carry the gene into the organism. According to this question, the correct order of the procedure is as follows:

- c. Isolate bacterial plasmid

- a. Select the gene of interest + insert into plasmid

- f. Plasmid taken up by bacterium

- d. Bacteria replicate

- e. Cloned gene harvested from bacteria

- b. Protein product harvested from bacteria